The Trenev Process

The Trenev Process® is a unique proprietary technology that ensures unmatched survivability and stability of the live probiotic bacteria. This cutting-edge technology uses an oil-matrix system to micro-enrobe the beneficial bacteria keeping them separated, non-competitive and virtually 100% protected from the stomach's gastric juices.

Bacteria are commonly antagonistic and will fight for survival, therefore multistrain probiotic products must be created in a way that maintains the separation of each individual strain. ProTren mixes together a maximum of only three organisms and uses this unique oil-matrix technology to keep them separate and non-competitive.

In addition to protecting the bacterial strains from one another, the oil-matrix also protects the live bacteria during their passage through the acidic stomach. This survivability has been validated through third-party testing by an internationally acclaimed European institute at pH 1.8 for 60-90 minutes.

The protective oil-matrix delivery system results in virtually 100% survivability, for over an hour in corrosive stomach acid, of the three probiotic bacteria found in ProTren's Trenev Trio and additional protection is provided by the two-piece hard gelatin capsule. 

MicroPure Technology

MicroPure Technology means you get a microbiologically pure and contaminant free probiotic.

Our temperature and humidity controlled facilities undergo rigorous testing to meet all current good manufacturing practices (cGMPs) established by the Food and Drug Administration (FDA).

Through a series of carefully developed steps, we protect the integrity of our probiotics from our culturing and manufacturing facility in Westlake Village, California, directly to you and your patients. ProTren probiotic products are manufactured and stored in a temperature and humidity-controlled environment, packaged in dark amber glass bottles, sealed with tin lids and shrink wrapped for added protection.

Genetic testing guarantees proper strain identification and Quality testing guarantees that the ingredients in ProTren probiotics:
• Remain Gluten-free
• Remain Soy-free
• Remain Dairy-free
• Contain no Genetically Modified Organisms (GMOs)
• Contain no Fructooligosaccharides (FOS).

ProTren does not use any ingredients produced by biotechnology. ProTren probiotic supplements are made only from natural ingredients, without soil-based organisms, hormones or artificial colors.

Thermally Controlled Vertical Manufacturing

Vertical Manufacturing means we own and produce our own probiotic products

ProTren is an experienced probiotic manufacturer that produces its probiotic supplements in its own state-of-the-art production facility in Westlake Village, California, USA. This facility maintains a proprietary full-culture and freeze drying process to preserve and nourish potent probiotics.

Our temperature and humidity controlled facilities undergo rigorous testing to meet all current good manufacturing practices (cGMPs) established by the Food and Drug Administration (FDA).

Probiotics are ALL that we do. Our seasoned technicians and management have hands-on control over the probiotics from the initial culturing steps through bottling and shipping. We are able to offer a level of focus and expertise that can only come with over three decades of continuous probiotic cultivation and production. With this focus comes a special care and determined innovation to deliver the most remarkable probiotic products available.

Thermally Controlled Custom Shipping

ProTren Intelligent Probiotics contain living bacteria; like most bacteria, they can be weakened or killed by prolonged exposure to moisture or heat. The bottles are each bubble-wrapped, not only for insulation but also to provide a moisture/freeze barrier between the probiotics and ice packs, then they are packaged carefully with air-bags for further protection during shipping.

A vital feature to shipping ProTren probiotic products cold is commercial grade, gel, ice packs and dry ice (when necessary in the warmer summer months). All of this is contained in a polystyrene layer within a cardboard outer box providing significant insulation from the outside environment.

Finally, the boxes are shipped on a strict shipping schedule (overnight or second-day air only) to optimize the speed at which they are delivered. We are so confident about our thermally controlled, custom shipping that we offer a 100% potency guarantee of each probiotic strain through the printed expiration date. That's the ProTren way of packaging and handling with the ultimate care.

All ProTren Precision Probiotics should be refrigerated upon arrival. We suggest that someone retrieve the package before the end of the day. We ship the products via UPS 2-day shipping in thermally controlled boxes. Once received, promptly refrigerate.

Clinical Trial Research

ProTren's probiotic products come with 30 years of focused, rigorous scientific research and technology from leading scientists and researchers in the field of probiotics. ProTren researchers have conducted and published a number of peer-reviewed clinical and laboratory studies on probiotic viability and application over these decades. Currently, ProTren is collaborating with several physicians on the applications of its probiotics in a variety of clinical studies.

No Artificial Colors or Genetically Modified Organisms

ProTren's probiotic supplements are made from only natural ingredients; without genetically modified organisms (GMOs), soil-based organisms, hormones, artificial colors, or FOS (Fructooligosaccharides). ProTren's proprietary Trenev Process® preserves the probiotics in their own natural supernatant to keep the probiotics healthy and viable. This intrinsic supernatant also provides nutritional support for the probiotics.*

Three Decades of Laboratory Research

ProTren's probiotic super-strains and formulations have undergone three decades of independent laboratory culturing and testing; resulting in some of the strongest and most powerful strains available. Bile tolerance and stomach acid survival tests have been conducted on each strain, and independent laboratories have confirmed ProTren's testing. Key super-strains produced by ProTren include the only Lactobacillus acidophilus, DDS-1 strain independently verified to be the strain isolated and tested by Dr. Shahani; the NAS super strain that integrates within the small intestines; the Bifidobacterium bifidum, Malyoth super strain that encourages large intestine wellness; the Lactobacillus bulgaricus, LB-51 super strain that assists digestive functions throughout the entire intestinal tract; and the LIFE START® bacteria, Bifidobacterium infantis, NLS.*

Research, Formulation & Production in the USA

Unlike the many brands that contract manufacture their probiotics elsewhere, ProTren is an experienced probiotic manufacturer that produces its probiotic supplements in its own state-of-the-art production facility in Westlake Village, California, USA. This pharmaceutical-grade product facility maintains a proprietary full-culture and freeze drying process to preserve and nourish vigorous beneficial probiotics.*

The Probiotic Specialist

Probiotics are ALL we do. ProTren's seasoned technicians and management have hands-on control over ProTren's probiotic products, with a level of focus and expertise that can only come with over three decades of continuous probiotics cultivation and production. With this focus comes a special care and determined innovation to deliver the most remarkable probiotic products available.*

Convenience & Reliability

ProTren has developed a variety of formulations and delivery systems. ProTren's formulations target specific areas or the entire digestive tract, and utilize different strains for different purposes. ProTren offers its variety of probiotic formulations in a host of innovative delivery systems, including chewable wafers, powders, capsules, creams and even yogurt starter.

Safety, Value & Customer Satisfaction

For over 30 years, ProTren's probiotics have been shown to be safe and have not produced significant side-effects when administered in large quantities. ProTren uses only lactic acid probiotic strains that have been used in fermented foods for centuries. Probiotics have been safely consumed by humans for thousands of years. Fermented foods have been associated with health and longevity amongst those societies that consumed them regularly.*

Authentic, Genuine & Passionate

Natasha Trenev's family introduced natural Bulgarian style yogurt and liquid acidophilus to the United States in the mid 1960's. Prior to World War II the family business was the official probiotic supplier to King Peter II of Yugoslavia. Each and every ProTren probiotic is infused with this healthy tradition and knowledge of beneficial bacteria. With this rich tradition also comes the ability to empower consumers with the wisdom of how to incorporate probiotics into their lives in the healthiest and most beneficial manner.*

Serious probiotics for people who take their health seriously.